3 hits
XFF4834R_chr15370 | rimJ | XFF4834R_chr15370 | probable ribosomal protein S5 alanine N-acetyltransferase |
XFF4834R_chr18550 | XFF4834R_chr19890270_2120683_f2_XFF4834R-XFF4834R_chr18550 | XFF4834R_chr18550 | hypothetical Iojap-like ribosome associated protein |
XFF4834R_chr21240 | engA | XFF4834R_chr21240 | probable GTP-binding protein EngA |
![]() XFF4834R_chr15360 |
![]() DrawMap |
XML file
FASTA file |
Extract Sequence |
Codon Usage |
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![]() XFF4834R_chr15380 |
ID | rimJ |
AC | XFF4834R_chr15370 |
LT | XFF4834R_chr15370 |
OR | XFF4834R_chr from 1770246 to 1770869 on strand + |
DE | probable ribosomal protein S5 alanine N-acetyltransferase |
IP | Acyl-CoA N-acyltransferase |
CL | 6.6 Ribosome GO:0009281 |
EC | |
GO |
InterPro Biological Process: metabolic process (GO:0008152) Molecular Function: N-acetyltransferase activity (GO:0008080) Curated |
PM | |
AN | |
CC | COG: [J] Translation, ribosomal structure and biogenesis; |
CC | MODEL: XFF4834R |
CC | STATUS: curated by cverniere (20100810) |
MW | 22956.1 Da |
SQ | 207 aa |
........10........20........30........40........50 |
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XFF4834R_chr41460 lcl|XFF4834R_chr_4822327_4822881_r1_XFF4834R-XFF4834R_chr41460 |
84 |
160 |
S=100 I=34 E=7.59582e-06 |
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NP_643404.1 lcl|rimJ-XAC3095 |
1 |
207 |
S=942 I=87 E=2.33668e-107 |
ribosomal protein alanine acetyltransferase |
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YP_364957.1 lcl|rimJ-XCV3226 |
1 |
207 |
S=884 I=83 E=2.38463e-100 |
ribosomal protein alanine N-acetyltransferase |
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NP_638266.1 lcl|rimJ-XCC2918 |
1 |
185 |
S=712 I=75 E=1.74206e-79 |
ribosomal protein alanine acetyltransferase |
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79 |
184 |
S=220 I=42 E=8.39374e-20 |
hypothetical acetyltransferase protein |
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NP_644579.1 lcl|XAC4280-XAC4280 |
84 |
160 |
S=99 I=34 E=3.58931e-05 |
acetyltransferase |
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31 |
188 |
S=266 I=38 E=8.03646e-24 |
Ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
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56 |
184 |
S=190 I=38 E=1.36951e-14 |
Probable acetyltransferase PacT |
Pubmed 8825087 |
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51 |
179 |
S=179 I=33 E=2.54123e-13 |
Probable acetylation of 30S S5 subunit |
Pubmed 15451099 |
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75 |
184 |
S=162 I=36 E=3.02029e-11 |
Putative uncharacterized protein |
Pubmed 15466567 |
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75 |
182 |
S=151 I=33 E=7.27985e-10 |
Uncharacterized N-acetyltransferase p20 EC=2.3.1.- ; Acyltransferase |
Pubmed 2838827 |
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31 |
177 |
S=312 I=46 E=1.36778e-29 |
Ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
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31 |
188 |
S=266 I=38 E=5.49597e-24 |
Ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
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31 |
188 |
S=266 I=38 E=5.49597e-24 |
Ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
||
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31 |
188 |
S=266 I=38 E=5.49597e-24 |
Ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
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33 |
184 |
S=262 I=36 E=1.70722e-23 |
Putative ribosomal-protein-alanine acetyltransferase EC=2.3.1.128 ; Acyltransferase Cytoplasm |
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IPR016181 [G3DSA:3.40.630.30] | 5 |
184 |
Acyl-CoA N-acyltransferase |
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IPR000182 [PF00583] | 80 |
157 |
GCN5-related N-acetyltransferase |
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IPR000182 [PS51186] | 29 |
183 |
GCN5-related N-acetyltransferase |
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[seg] | 12 |
30 |
- |
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101 |
141 |
S=204 I=98 E=2.88369e-17 |
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184 |
207 |
S=113 I=92 E=3.03714e-06 |
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142 |
188 |
S=106 I=43 E=2.06774e-05 |
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IPR016181 [SSF55729] | 1 |
184 |
Acyl-CoA N-acyltransferase |
Access unfiltered results
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Set of Uniprot with relevant PubMed cross-references ncbi-blastp |
6 hits |
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Swiss-Prot ncbi-blastp |
8 hits |
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Xanthomonas fuscans subsp. fuscans proteome ncbi-blastp |
1 hits |
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HAMAP scan |
0 hits |
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IprScan |
5 hits |
Miscellaneous analyses
![]() XFF4834R_chr18540 |
![]() DrawMap |
XML file
FASTA file |
Extract Sequence |
Codon Usage |
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![]() XFF4834R_chr18560 |
ID | XFF4834R_chr19890270_2120683_f2_XFF4834R-XFF4834R_chr18550 |
AC | XFF4834R_chr18550 |
LT | XFF4834R_chr18550 |
OR | XFF4834R_chr from 2120270 to 2120683 on strand + |
DE | hypothetical Iojap-like ribosome associated protein |
IP | Iojap-related protein |
CL | 6.6 Ribosome GO:0009281 |
EC | |
GO |
InterPro Curated |
PM | |
AN | Iojap-like protein = widely distibuted family probably associated with ribosomes but with unknown functions so hypothetical Iojap-like protein |
CC | COG: [S] Function unknown; |
CC | MODEL: XFF4834R |
CC | STATUS: curated by bonneau (20100929) |
MW | 15216.2 Da |
SQ | 137 aa |
........10........20........30........40........50 |
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YP_364664.1 lcl|XCV2933 |
1 |
136 |
S=621 I=90 E=1.088e-68 |
hypothetical protein |
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NP_643086.1 lcl|XAC2777-XAC2777 |
1 |
136 |
S=621 I=90 E=1.088e-68 |
hypothetical protein |
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NP_637963.1 lcl|XCC2615 |
1 |
133 |
S=576 I=85 E=3.19406e-63 |
hypothetical protein |
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1 |
132 |
S=460 I=76 E=3.18458e-49 |
hypothetical iojap-related protein |
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20 |
121 |
S=223 I=41 E=5.46855e-19 |
Uncharacterized protein ybeB |
Pubmed 3533535 |
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37 |
120 |
S=115 I=31 E=6.05873e-06 |
YqeL protein |
Pubmed 15558816 |
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20 |
121 |
S=223 I=41 E=3.85333e-19 |
Uncharacterized protein ybeB |
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20 |
121 |
S=223 I=41 E=3.85333e-19 |
Uncharacterized protein ybeB |
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20 |
121 |
S=223 I=41 E=3.85333e-19 |
Uncharacterized protein ybeB |
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21 |
120 |
S=188 I=37 E=5.44309e-15 |
Uncharacterized protein HI0034 |
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34 |
122 |
S=155 I=39 E=6.57201e-11 |
Uncharacterized protein C7orf30 |
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IPR004394 [PF02410] | 22 |
120 |
Iojap-related protein |
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IPR004394 [TIGR00090] | 17 |
120 |
Iojap-related protein |
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[seg] | 94 |
105 |
- |
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1 |
52 |
S=244 I=88 E=4.45938e-22 |
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53 |
121 |
S=112 I=44 E=3.72845e-06 |
Access unfiltered results
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Set of Uniprot with relevant PubMed cross-references ncbi-blastp |
2 hits |
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Swiss-Prot ncbi-blastp |
7 hits |
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Xanthomonas fuscans subsp. fuscans proteome ncbi-blastp |
0 hits |
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HAMAP scan |
0 hits |
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IprScan |
3 hits |
Miscellaneous analyses
![]() XFF4834R_chr21230 |
![]() DrawMap |
XML file
FASTA file |
Extract Sequence |
Codon Usage |
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![]() XFF4834R_chr21250 |
ID | engA |
AC | XFF4834R_chr21240 |
LT | XFF4834R_chr21240 |
OR | XFF4834R_chr from 2463697 to 2465094 on strand + |
DE | probable GTP-binding protein EngA |
IP | GTP-binding protein, HSR1-related; ATPase, AAA+ type, core; Small GTP-binding protein |
CL | 6.6 Ribosome GO:0009281 |
EC | |
GO |
InterPro Cellular Component: intracellular (GO:0005622) Molecular Function: nucleoside-triphosphatase activity (GO:0017111) Molecular Function: GTP binding (GO:0005525) Molecular Function: nucleotide binding (GO:0000166) Curated |
PM | |
AN | engA (der) is annotated 50S ribosome-associated factor, involved in ribosome biogenesis in GenProtEC Hwang and Inouye 2006: A unique GTP-binding protein, Der contains two consecutive GTP-binding domains at the N-terminal region and its homologues are highly conserved in eubacteria but not in archaea and eukaryotes. In the present paper, we demonstrate that Der is one of the essential GTPases in Escherichia coli and that the growth rate correlates with the amount of Der in the cell. Interestingly, both GTP-binding domains are required at low temperature for cell growth, while at high temperature either one of the two domains is dispensable. Result of the sucrose density gradient experiment suggests that Der interacts specifically with 50S ribosomal subunits only in the presence of a GTP analogue, GMPPNP. The depletion of Der accumulates 50S and 30S ribosomal subunits with a concomitant reduction of polysomes and 70S ribosomes. Notably, Der-depleted cells accumulate precursors of both 23S and 16S rRNAs. Moreover, at lower Mg2+ concentration, 50S ribosomal subunits from Der-depleted cells are further dissociated into aberrant 50S ribosomal subunits; however, 30S subunits are stable. It was revealed that the aberrant 50S subunits, 40S subunits, contain less ribosomal proteins with significantly reduced amounts of L9 and L18. These results suggest that Der is a novel 50S ribosome-associated factor involved in the biogenesis and stability of 50S ribosomal subunits. We propose that Der plays a pivotal role in ribosome biogenesis possibly through interaction with rRNA or rRNA/r-protein complex. Bharat et al 2006: To probe the cellular phenotype and biochemical function associated with the G domains of Escherichia coli EngA (YfgK, Der), mutations were created in the phosphate binding loop of each. Neither an S16A nor an S217A variant of G domain 1 or 2, respectively, was able to support growth of an engA conditional null. Polysome profiles of EngA-depleted cells were significantly altered, and His(6)-EngA was found to cofractionate with the 50S ribosomal subunit. The variants were unable to complement the abnormal polysome profile and were furthermore significantly impacted with respect to in vitro GTPase activity. Together, these observations suggest that the G domains have a cooperative function in ribosome stability and/or biogenesis. |
CC | COG: [R] General function prediction only; |
CC | MODEL: XFF4834R |
CC | STATUS: curated by darrasse (20120307) |
MW | 51324.8 Da |
SQ | 465 aa |
........10........20........30........40........50 |
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ENGA_MF_00195 | raw_score=9509 norm_score=39.761 | GTP-binding protein engA [engA]. |
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XFF4834R_chr42940 lcl|mnmE-XFF4834R_chr42940 |
183 |
288 |
S=161 I=38 E=7.84264e-13 |
GTPase |
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XFF4834R_chr42940 lcl|mnmE-XFF4834R_chr42940 |
7 |
100 |
S=112 I=32 E=7.66855e-07 |
GTPase |
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XFF4834R_chr31750 lcl|era-XFF4834R_chr31750 |
5 |
61 |
S=126 I=46 E=1.52958e-08 |
membrane-associated, 16S rRNA-binding GTPase |
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XFF4834R_chr31750 lcl|era-XFF4834R_chr31750 |
181 |
289 |
S=122 I=37 E=4.16889e-08 |
membrane-associated, 16S rRNA-binding GTPase |
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XFF4834R_chr27750 lcl|hflX-XFF4834R_chr27750 |
2 |
163 |
S=119 I=35 E=8.59608e-08 |
predicted GTPase |
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XFF4834R_chr20000 lcl|feoB-XFF4834R_chr20000 |
2 |
123 |
S=103 I=30 E=9.69585e-06 |
fused ferrous iron transporter, protein B: GTP-binding protein/membrane protein |
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NP_642347.1 lcl|engA-XAC2021 |
1 |
465 |
S=2335 I=96 E=0 |
GTP-binding protein EngA |
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YP_363803.1 lcl|engA-XCV2072 |
1 |
465 |
S=2331 I=96 E=0 |
GTP-binding protein EngA |
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NP_637352.1 lcl|engA-XCC1987 |
1 |
465 |
S=2319 I=95 E=0 |
GTP-binding protein EngA |
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1 |
465 |
S=2065 I=83 E=0 |
probable gtp-binding protein enga. |
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NP_639572.1 lcl|trmE-XCC4238 |
183 |
288 |
S=164 I=38 E=1.23583e-12 |
tRNA modification GTPase TrmE |
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NP_639572.1 lcl|trmE-XCC4238 |
7 |
100 |
S=114 I=32 E=1.52913e-06 |
tRNA modification GTPase TrmE |
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YP_366214.1 lcl|trmE-XCV4483 |
183 |
288 |
S=162 I=38 E=2.29509e-12 |
tRNA modification GTPase TrmE |
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YP_366214.1 lcl|trmE-XCV4483 |
7 |
100 |
S=112 I=32 E=2.62547e-06 |
tRNA modification GTPase TrmE |
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NP_644664.2 lcl|trmE-XAC4370 |
183 |
279 |
S=160 I=38 E=3.83887e-12 |
tRNA modification GTPase TrmE |
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NP_644664.2 lcl|trmE-XAC4370 |
7 |
100 |
S=111 I=32 E=3.47037e-06 |
tRNA modification GTPase TrmE |
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1 |
463 |
S=1137 I=47 E=6.23901e-129 |
Putative GTP-binding protein ; Nucleotide-binding Repeat |
Pubmed 15313254 |
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1 |
458 |
S=1001 I=46 E=2.14182e-112 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 10655208 |
|
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1 |
451 |
S=995 I=45 E=1.0936e-111 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 19077236 |
|
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1 |
444 |
S=890 I=38 E=5.77453e-99 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 9516544 |
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3 |
435 |
S=847 I=41 E=8.43936e-94 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 18441057 |
|
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3 |
435 |
S=842 I=40 E=3.20365e-93 |
GTP-binding protein ; Nucleotide-binding Repeat |
Pubmed 12446909 |
|
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177 |
347 |
S=185 I=32 E=1.6277e-13 |
GTP-binding protein ; Nucleotide-binding Repeat |
Pubmed 12446909 |
|
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3 |
434 |
S=800 I=39 E=4.18035e-88 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 18263572 |
|
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177 |
344 |
S=189 I=32 E=4.84452e-14 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 18263572 |
|
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3 |
434 |
S=731 I=35 E=9.18086e-80 |
GTP-binding protein engA ; Nucleotide-binding Repeat |
Pubmed 18281406 |
|
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[G3DSA:3.40.50.300] | 5 |
168 |
- |
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[G3DSA:3.40.50.300] | 178 |
361 |
- |
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IPR016484 [MF_00195] | 2 |
437 |
GTP-binding, EngA |
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IPR016484 [PIRSF006485] | 1 |
441 |
GTP-binding, EngA |
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IPR002917 [PF01926] | 3 |
122 |
GTP-binding protein, HSR1-related |
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IPR002917 [PF01926] | 179 |
300 |
GTP-binding protein, HSR1-related |
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IPR003593 [SM00382] | 1 |
148 |
ATPase, AAA+ type, core |
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IPR003593 [SM00382] | 177 |
325 |
ATPase, AAA+ type, core |
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IPR005225 [TIGR00231] | 1 |
161 |
Small GTP-binding protein |
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IPR016484 [TIGR03594] | 3 |
434 |
GTP-binding, EngA |
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IPR005225 [TIGR00231] | 176 |
346 |
Small GTP-binding protein |
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[seg] | 69 |
83 |
- |
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37 |
100 |
S=229 I=75 E=2.97128e-20 |
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367 |
438 |
S=181 I=46 E=2.33632e-14 |
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341 |
370 |
S=150 I=97 E=1.16936e-10 |
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436 |
465 |
S=136 I=87 E=6.50919e-09 |
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226 |
364 |
S=135 I=28 E=9.14535e-09 |
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224 |
273 |
S=129 I=50 E=4.92035e-08 |
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341 |
370 |
S=117 I=77 E=1.10606e-06 |
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7 |
40 |
S=115 I=65 E=2.09022e-06 |
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183 |
220 |
S=113 I=61 E=3.71623e-06 |
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176 |
247 |
S=109 I=37 E=1.22703e-05 |
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436 |
461 |
S=107 I=77 E=1.89749e-05 |
Access unfiltered results
Miscellaneous analyses